Questionable Reliability of Malondialdehyde to Measure Oxidative Stress in Sjogren’s Syndrome: Preliminary Study
이경은, 정원, 서봉직, 차승희,
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이경은 ( Lee Kyung-Eun ) - Chonbuk National University School of Dentistry Department of Oral Medicine
정원 ( Jung Won ) - Chonbuk National University School of Dentistry Department of Oral Medicine
서봉직 ( Suh Bong-Jik ) - Chonbuk National University School of Dentistry Department of Oral Medicine
차승희 ( Cha Seung-Hee ) - University of Florida College of Dentistry Department of Oral and Maxillofacial Diagnostic Sciences
Abstract
Purpose: To investigate the expression of malondialdehyde (MDA), lipid peroxidation marker for oxidative stress (OS), in autoimmune Sjogren’s syndrome (SjS) by utilizing the SjSprone C57BL/6.NOD-Aec1Aec2 (B6DC) mouse and the SjS patient plasma samples.
Methods: The MDA concentrations in the lysates of the submandibular gland, liver, and serum samples from the SjS-prone B6DC mouse model were compared with those from the C57BL/6J as a control. A thiobarbituric acid reactive substance (TBARS) assay kit was used to measure MDA. Plasma samples from five SjS patients and five control subjects were also evaluated.
Results: The MDA concentrations in experimental animals and controls were not significantly different. There were no significant differences between the plasma of SjS patients and of controls.
Conclusions: The expression of MDA was investigated in the organs from the SjS-prone B6DC mouse for the first time and in the plasma samples of SjS patients. No significant differences were observed between SjS and control samples when MDA was the target molecule with the TBARS assay. MDA may not be a reliable marker to measure OS contrary to the published studies involving OS of SjS.
키워드
C57BL/6.NOD-Aec1Aec2; Malondiadehyde; Oxidative stress; Sjogren’s syndrome
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